Storage lactate dehydrogenase

Studies of release of noradrenaline from sympathetic neurons provided the first convincing evidence that impulse (Ca +)-dependent release of any transmitter depended on vesicular exocytosis. Landmark studies carried out in the 1960s, using the perfused cat spleen preparation, showed that stimulation of the splenic nerve not only led to the detection of noradrenaline in the effluent perfusate but the vesicular enzyme, DpH, was also present. As mentioned above, this enzyme is found only within the noradrenaline storage vesicles and so its appearance along with noradrenaline indicated that both these factors were released from the vesicles. By contrast, there was no sign in the perfusate of any lactate dehydrogenase, an enzyme that is found only in the cell cytosol. The processes by which neuronal excitation increases transmitter release were described in Chapter 4. 

Miller et al. [3.83] freeze-dried lactate dehydrogenase (LDH) in the presence of trehalose and trehalose plus sodium tetraborate (TST) to stabilize LDH for storage at high humidity (100%) or warm temperature (45 °C). The freeze-dried LDH with TST had a considerably higher Tg than with trehalose alone and was more stable for several weeks under the conditions given above. 

There are two additional postulated Mb functions that should be mentioned. First, Mb may serve as a simple intracellular 02 store. In muscles of marine mammals, Mb occurs at 8-12 times higher concentrations than in muscles of most other species, and it is in these kinds of settings that Mb is thought to play an intracellular 02 storage function (Hochachka and Foreman, 1994). Additionally, there is a linear relationship between the anaerobic capacity of muscles (as indicated by the amounts of lactate dehydrogenase, or LDH, that they contain) and their Mb content. This relationship probably arises because protons generated by anaerobic glycolysis may be buffered by intracellular Mb,... 

Effect of Type of Amorphous Sugar Excipients on the Preservation of Lactate Dehydrogenase Activity as a Function of Storage Conditions... 

Residual activity of lactate dehydrogenase after 30 days storage. 

Nambudiri, D.D. and Gopakumar, K. 1992. ATPase and lactate dehydrogenase activities in frozen stored fish muscle as indices of cold storage deterioration. Journal of Food Science 57 72-76. 

Glycogen storage disease Myophosphoiylase Glycerol kinase Lactate dehydrogenase Phospholni inase Phosphoglycerate kinase Myoadenylate deaminase... 

Response times for the lactate dehydrogenase biosensor in either the lactate or pyruvate sensing mode range from 6 to 12 minutes. Faster response is obtained with higher concentrations. In addition, the lifetime of this sensor is from 3 to 7 days depending on the extent of enzyme loading and the storage conditions. 

Glycogen storage disease type XI Lactate dehydrogenase (LDH) LDHA ... 

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