Sterilization moist

Because of the obligate outcrossing nature of alfalfa, all experiments were carried out using vegetative cuttings of primary transformants. Nodal stem sections were placed in sterile, moist vermiculite for approx 14 d to stimulate adventitious root production, then transferred to a mix of sand and soil (1 1 [v/v]) in 3.8 x 21 cm plastic cone-tainers (Stuewe Sons, Corvallis, OR) and fertilized monthly with soluble 10 10 10 (N P K) fertilizer. 

Statistical Process Control and Process Capability / 3499 Sterilization Dry Heat / 3512 Sterilization Ethylene Oxide / 3519 Sterilization Moist Heat / 3529 Sterilization Radiation / 3540... 

Thirty-one isolates of Fusarium from sorghum were screened for ZE and DON production by the thin-layer chromatographic method of Richardson et al. (1984). Four replicate cultures (sterile moist rice) of each isolate were examined. Cultures were incubated at 25-27 C under 12 hr fluorescent Iight/12 hr darkness for 30 days. Cultures were then analyzed for ZE and DON. 

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