Steady state, enzymatic reactions before

The initial rate enzyme kinetics uses very low enzyme concentrations (e.g., 0.1 juM to 0.1 pM) to investigate the steady-state region of enzyme-catalyzed reactions. To investigate an enzymatic reaction before the steady state (i.e., transient state), special techniques known as transient kinetics (Eigen and Hammes, 1963) are employed. The student should consult chapters of kinetic texts (Hammes, 1982 Robert, 1977) on the topics. KinTekSim (http //www.kintek-corp.com/kintek-sim.htm) is the Windows version of KINSIM/FITSIM (Frieden, 1993) which analyzes and simulate enzyme-catalyzed reactions. 

To determine individual rate constants (i.e., k and k-i) for the mechanism depicted above, it is necessary to monitor the progress of the reaction before establishment of the steady state. This pre-steady-state region of an enzymatic reaction is called the transient phase of an enzymatic reaction. For this purpose, it is necessary to carry measurements of a single turnover of substrate into product, usually using enzyme concentrations in the range of those of substrate ([E] [S]). 

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