Staining agarose embedded DNA

Staining a rose embedded DNA 4.2.1 Embedding and i ing ceils in agarose on microscf pe slides [Pg.427]

Microscope slide staining tanks and slide carriers [Pg.427]

Coat the microscope slides with a thin layer of agarose. Melt an aliquot of 0.5% agarose in a boiUng water-bath and spread a drop of the solution along each slide using the emi of a second slide indined at an angle of about 45 [Pg.427]

Melt an aliquot of 2% agarose gel in a boiling water-bath and equilibrate to S [Pg.427]

After a few minutes to allow the agarose to set, gentfy immerse the slide in tiie lyas solution in a slide staining tank and leave for at least 5 min at room temperature. [Pg.427]

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