Staggered sequence

The example cited required 85 C—typically a waste heat temperature for most industrial environments or a temperature readily delivered by solar heating installations. There are no rotating parts requiring maintenance. Check valves regulate the hydrogen flow. Continuous operation is achieved by using multiple hydride beds operated in a staggered sequence. 

The POLYBED PSA hydrogen system uses five or more adsorbers in a staggered sequence. Several adsorbers are on adsorption steps at any time while the others are in various stages of regeneration. The different cycle steps which an adsorber experiences during one complete cycle are summarized below and in Table I. 

In a typical adsorption process, a multitude of adsorbent beds are operated in a cyclic manner, with each bed repeatedly undergoing the same steps but in a staggered sequence so that a continuous flow can be maintained. Adsorption is an inherently dynamic process and suffers from the disadvantages associated with cyclic operation. 

When coRI encounters this sequence in dsDNA, it causes a staggered, double-stranded break by hydrolyzing each chain between the G and A residues ... 

Step through the sequence of structures depicting bond rotation in ethane. Plot energy (vertical axis) vs. HCCP torsion angle (horizontal axis). Do the minima correspond to staggered structures Do the maxima correspond tc eclipsed structures If not, to what do they correspond ... 

Terminal deoxynucleotidyl transferase normally adds homopolydeoxynucleotide tails to single-stranded DNA primers in the presence of a deoxynucleoside triphosphate and magnesium. If cobalt is used instead, not only does double-stranded DNA become an acceptable substrate, but ribonucleotides or homopolymer deoxyribo-nucleotide tracts may be added to all forms of duplex DNA at their 3 -ends, regardless of whether these are staggered or even.162 This allows terminal labelling for sequence analysis at the cleavage sites of restriction endonucleases,162- 183 or tail formation for in vitro studies on recombinant DNA.162... 

Restriction enzymes are sequence-specific in that they cut DNA at specific locations along the nucleotide chain. While some of these enzymes yield "blunt" ends to the resulting DNA fragment, others make staggered cuts in the DNA chain to produce "sticky" ends. Over 250 restriction enzymes are now commercially available. 

The EcoRI endonuclease cleaves this hexanucleotide sequence on each strand between the G and the first A residue (reading 5 -to-30 this point of cleavage is marked by arrows in the sequences in Table 3.1. Notice that the points of cleavage are offset, or staggered, for EcoRI. Not all restriction endonucleases make staggered cuts in DNA some cleave the DNA without any offset, to produce blunt-ended cuts. 

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