Stable transgene expression

CPMP (2002) Points to consider on quality aspects of medicinal products containing active substances produced by stable transgene expression in higher plants (CPMP/BWP/764/... 

CPMP/BWP/764/02 Points to Consider on Quality Aspects on Medicinal Products Containing Active Substances Produced by Stable Transgene Expression in Higher Plants (CPMP adopted April 2002). 

Tab. 12.2 Transgene expression is stable over eight generations and is independent of growth...

Ledesma, A., Moral, V., Villalba, M., Salinas, J., and Rodriguez, R. (2(X)6). Ca + binding allergens from olive pollen exhibit biochemical and immunological activity when expressed in stable transgenic Arabidopsis. FEBS 273(19) 425-434. 

Four basic strategies for recombinant protein expression in plants are transient expression, stable nuclear transformed plants, chloroplast transformed plants, and suspension cultures derived from stable transgenic lines. 

To establish efficient transgene expression, the ssDNA genome of the vectors has to be converted into a dsDNA, and this appears to be the rate-limiting step in AAV transduction (19). Unless the ssDNA genome is converted into a stable dsDNA genome, it is lost rapidly after transduction. Because transduction occurs in the absence of helper vims and rep proteins, AAV relies solely on cellular conditions supporting this event. When the ssDNA is converted into dsDNA by the host cell machinery, transgene expression is increased concomitantly (20,21). 

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