Stable Isotope Standards and Capture by Anti-Peptide Antibodies SISCAPA

Anderson NL, Anderson NG, Haines LR, et al. Mass spectrometric quantitation of peptides and proteins using stable isotope standards and capture by anti-peptide antibodies (SISCAPA)./. Proteome Res. (2004) 3 235-244. 

Immunoenrichment can be applied at the peptide level, particularly in the stable isotope standards and capture by anti-peptide antibodies (SISCAPA) method (140). Target peptides together with their stable isotope-labeled counterparts (used as quantitation references) are enriched by antibodies that are developed against antigens with almost the same sequences. The antibodies are often covalently attached to magnetic beads for sample cleanup. This technique boasts an improved limit of quantitation and increased inter-laboratory reproducibility (141). 

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