Stability constants calcium-binding proteins

Approximate Stability Constants, Logiq-ST, for Some Calcium-Binding Proteins... 

Some 13 years after Carr s original observations, Kretsinger (1976), in his review of calcium-binding proteins, assumed that lysozyme can attach Ca(ll), as well as other cations. It was not until 1981 that binding of Ca(Il) to lysozyme was further studied. Imoto et al. (1981) determined the stability (association) constant (40 M" ) and found that lysozyme is inhibited in the presence of Ca(II), showing only 26% of the activity of the free enzyme toward hexa-AT-acetylglucosamine. Because of this inhibition, they predicted that Ca(II) binds near the catalytic carboxyls. Furthermore, Ca(II) shifts the native-denatured transition in lysozyme toward the native state, and thus has some preservative effect on the protein. 

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