Squirt bottle

Rinse with SC buffer from squirt bottle. 

The volnme of PBS used in washes is not critical. It may be applied from a squirt bottle, and the well may be filled (about 2 mL). The animal will sink in the PBS solution when all of the ethanol has been removed from the tissne (1-2 min). Before each incubation period, the animal should be checked to make sure it is submerged in the solution. If it floats on the surface of the fluid, the tissue not exposed to the fluid will not be fixed or, in later steps, labeled with reagent. 

Rinse the grids in a stream of deionized glass-distilled water from a squirt bottle and dry. 

Step 24. Remove the electrode from the cell and turn off the power. Promptly use transfer pipette to discard the solution. Rinse the disk in the cell with 1% solution of ammonium nitrate in 1 99 ammonium hydroxide from a squirt bottle. Discard rinse. 

Puromycin solution (0.1 mM) in distilled water—Sigma catalog P-7255 Plastic squirt bottle... 

Using a fine-tipped forceps, place a 0.45-pm HAWP filter in the filtration apparatus (to be demonstrated by the instructor). Fill a squirt bottle with 5 % TCA and apply a small volume to the filter to moisten it. 

To remove excess probe, heat hybridization wash buffer (0.1X SSC/ 0.1% SDS, 600-750 mL/filter total) to 60-65°C. Remove the membranes from the hybridization mix, rinse each one with 5-10 mL of wash buffer from a squirt bottle, and place them immediately in a container of wash buffer (200-250 mL/filter). Two or three membranes may be washed together. Do not let membranes dry before washing is complete. 

Incubate the membranes in wash buffer (250 mL/membrane) for 20 min at 60-65°C. Pour off wash buffer (into an appropriate container). Repeat the incubations, washing membranes a total of three times. Turn membranes over after each wash. Rinse the membranes with distilled H20 (in a squirt bottle) after the final wash and air-dry (5-10 min). 

Spraying the combs with 2% SDS (in H20) with a squirt bottle makes it easier to remove the combs from the agarose without producing holes in the bottoms of the wells. 

Gently cleanse the perineum with a squirt bottle and tepid water after urinating. 

Rinse twice with PBS (use squirt bottle and direct jet onto side of well). 

Squirt bottle, 500-mI capacity with rapid stream Dry ice/95% ethanol bath in an insulated container 600-ml beaker Pasteur pipets... 

Wash embryos off of food plates through nested stainless-steel sieves (Bellco Glass Inc.) using a stream of wash solution from a squirt bottle, and a paintbrush if necessary. The upper sieve traps flies and other debris, and the lower sieve retains the embryos. 

Pour the embryos back through the finer sieve, and rinse extensively with a stream of wash solution from a squirt bottle to remove the bleach. Rinse the embryos into one side of the sieve. 

Ifou go into the laboratory to look for a squirt bottle containing acetone. i>u find two unlabeled bottles with different colored tops suggesting they are different liquids. Unfortunately, you have a terrible cold and cannot teU by smell which one might be acetone. What simple test could you use to determine which liquid is acetone and which is water How does this test tell you this information ... 

When you use a squirt bottle, never touch the tip to anything. 

After 20 minutes, add a few milliliters of de-ionized water to the material in the mortar. Scrape the mortar and pestle a final time to loosen all of the product from the mortar. Pour the mixture into a Hirsch funnel inserted into a filter flask under vacuum. Use a squirt bottle with de-ionized water to transfer any remaining off-white product into the Hirsch funnel. Discard the filtrate that contains potassium phosphate and some triphenylphosphine oxide. The off-white solid consists mainly of the trans,trans isomer, but some of the cis,trans isomer will be present, as well. 

Dimethylformamide (DMF). Reagent-grade DMF is adequate for resin washing however, it should be stored over activated 4 A molecular sieves for at least 24 h prior to use as the coupling reaction solvent to remove water and dimethylamine. For manual synthesis, fill a 500 mL squirt bottle with DMF for rapid resin washing. Dichloromethane (DCM). For manual synthesis, fill a 500 mL squirt bottle as for DMF. 

The objective of rinsing the electrodes between measurements is to prevent contamination by carry-over on the electrodes. It has become common practice to use distilled water from a squirt bottle to rinse the electrodes and then to blot them with a tissue. This practice, however, has led to the improper technique of wiping rather than blotting the electrodes. Wiping often results in slower responding electrodes, thus requiring considerable time before a stable pH reading is obtained. 

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