Splitless injection matrix effects

Splitless injection involves keeping the injector split vent closed during the time the sample is deposited on the column, after which the vent is reopened and the inlet purged with carrier gas. In splitless injection, the inlet temperature is elevated with respect to the column temperature. The sample is focused at the head of the column with the aid of the solvent effect. The solvent effect is the vaporization of sample and solvent matrix in the injection port, followed by trapping of the analyte in the condensing solvent at the head of the column. This trapping of the analyte serves to refocus the sample bandwidth and is only achieved after proper selection of the solvent, column and injector temperatures. Splitless injection techniques have been reviewed in References 29 and 30. 

Splitless injection high injector temperature improves sample transfer and reduces matrix effects. 

Splitless injection a light plug of glass wool reduces matrix effects for solutes having fairly high boiling point. 

The most common injection methods in the determination of FRs are splitless injection and on column injection. On column injection is suitable especially to thermally labile analytes, and it suits very well to quantitative analysis. However, the sample extract should be clean from nonvolatile matrix components in on column injection. Split injection is not recommended because of its low sensitivity and strong discrimination effects which can occur during the injection. Large volume injection techniques have also been applied in the analysis of FRs. ... 

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