Split-hybrid system

Fig. 7.4. Variations of the original Y2H assay have been designed to detect the disruption of protein-protein interactions. A. In the reverse Y2H system, a direct protein-protein interaction between X and Y activates the transcription of URA3, which converts 5-FOAto a toxic compound (left) however, if the interaction between X and Y is disrupted, then transcription of URA3 is disrupted and the cells can grow in media containing 5-FOA (right) [28]. B. In the split-hybrid system, a direct protein-protein interaction between X and Y ac-...

MESH) equations which are solved for the whole column, decanter included and taking into account the liquid-liquid phase split. Numerical treatment of the Differential Algebraic Equation (DAE) system and discrete events handling is performed with DISCo, a numerical package for hybrid systems with a DAE solver based on Gear s method. The column technical features and operating conditions are shown in Table 4. A sequence of two operational batch steps, namely... 

Thaminy, S., Auerbach, D., Amoldo, A. and Stagljar, 1. (2003) Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res. 13, 1744-1753. 

It can be seen that once an r i-value is set, all the values in Table 9.1 are known. However, setting r i alone does not fuUy specify the entire hybrid system. Another degree of freedom is the amount of liquid that enters the membrane unit from the end of CSi. Only liquid is drawn off since a pervaporation membrane is incorporated. The split ratio. 

In specific cases when the vapor-liquid equilibrium favors distillation at the side of the organic component of an azeotrope and a high purity of the organic component is specified the membrane system may be used just to split the azeotrope. For the separation of the system acetonitrile-water a hybrid system as shown in Fig. 3.19 may be economically advantageous. Here the membrane system is used to cross the azeotropic point the partially dehydrated vapor enters the second column in which final dehydration is effected. Again it is necessary to determine the economical optimum between the size of both columns, the energy consumption of the first one and the volume of the recycle stream from the second column at one side, and the size of the membrane system and its outlet concentration on the other side. 

This metal leaf may eventually turn sunlight and water into. .. The most recent advance in this area at press time combines this with microbes J. P. Torella et al. Efficient solar-to-fuels production from a hybrid microbial-water-splitting catalyst system. 2015. Proc NatlAcadSci USA. 112(8),p. 2337. DOI 10.1073/pnas.l424872112. 

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