Spinning cell

Discard the supernatant, resuspend the cells pellet in 4 ml of ice-cold lysis buffer, and spin cells again as in step 2. 

Collect the cells and spin cell at 1500 rpm for 10 min at room temperature. 

Eysel and Bertie (1988) considered the absolute normalized Raman scattering cross section of the ring breathing vibration of liquid benzene at 992 cm a standard for the measurement of relative Raman scattering cross sections of a number of compounds. When applying it as standard they made use of a divided spinning cell , developed by Kiefer and Topp (1974). 

Nanosecond pump probc time-resolved resonance Raman experiments were carried out with two Nd YAG lasers (At=7 ns) which provide the pump- (532 nm, 4 mJ) and probe pulses (416 nm, 100 mJ), a triple polychromator equipped with an intensified photodiode array, and a slow spinning cell (25tpm). For every sample, spectra were taken for delay times of At=-20, 0, 20,100 and 500 ns, 1,10,100 and 500 is, and 1ms. ... 

Interfacial Tension Measurements. A Site Model LP-10 Spinning Drop Interfacial Tensiometer was used in this study. A description of the instrument and its capabilities have been reported (25-28). The apparatus consists of four major components the spinning cell, the... 

Separate the induced yeast into several samples. Spin cells down at 3,000rpm (800xg) for 3min and aspirate the supernatants. Resuspend each sample in an appropriate volume of PBS/0.5% BSA (see Subheading 3.2.5, step 4b). 

Fig. 4. Experimental arrangement for transient RR and TR studies using a spinning cell. The chopper and electronic switch are needed only for high rotational speeds so that the resting state has been recovered. Bottom) Arrangement for the case where two beams (pump and probe) are used. (From Lohrman and Stockburger and Diller and Stockburger. )...

It is noteworthy that the 0-0 stretching Raman band of oxygenated cytochrome P-450 was identified at 1140 cm (1074 cm for 02) by using a spinning cell at... 

Early studies have found that PVA is soluble in liquid ammonia solutions of 15 55% PVA content were ejected from the spinneret into the spinning cell with its own pressure of about 8 atm and at room temperature. Take-up speed of the fiber was 60 270 m/min [136]. 

Cobalt complexes are the specific area of interest for a paper dealing with the technology of spinning-cell Fourier transform Raman spectroscopy. The technique uses near-IR light and, because of the rapidly spinning cell, avoids the problems of sample burning and should be most useful for the study of delicate carbonyl species. Clusters are studied somewhat differently, following UV laser photolysis, in a paper published by Belyaev et. ... 

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