Sphingomyelin hydrolysis studies

Kwon et al. (1996) used normal-phase HPLC to separate and quantify sphingomyelin (SPH). Flow injection ES-MS was then used to determine the SPH molecular species composition of isolated rat pancreatic islets. Kwon et al. concluded that sphingomyelin hydrolysis is not involved in the signalling pathway whereby cytokine interleukin-1 induces the overproduction of nitric oxide by pancreatic islets. They reported four molecular species of SPH SPH (16 0/d-18 l), (18 0/d-18 l), (22 0/d-18 l) and (24 0/d-18 l). They assumed that sphingosine (18 1) is the LCB however, studies of SPH from bovine brain (Jungalwala, Evans and McCluer, 1984), bovine milk (Morrison, 1969) and human plasma (Sweeley, 1963), show that there exist several LCB species, saturated as well as unsaturated, with one or two hydroxyl... 

Zhang et al. studied a membrane reactor for the production of ceramide through sphingomyelin hydrolysis with phospholipase C from Clostridium perfringens. Ceramide has aroused considerable interest as an active component in both the pharmaceutical and the cosmetic industry. The enzymatic hydrolysis of sphingomyelin has proved a feasible method for the production... 

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