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Sphingolipids analysis

Our new method of sphingolipid analysis using high performance liquid chromatography allows us to determine not only their quantities but also their homolog compositions in a small amount of tissue. We now feel that less than 1 mg of fresh brain or nerve tissue is sufficient for complete analysis. The application of this new method for analyzing cerebroside and sulfatide-in plaques of brain from a patient with multiple sclerosis has been recently described (15). [Pg.28]

Bartke, N., Fischbeck, A., Humpf, H. U. (2006). Analysis of sphingolipids in potatoes Solanum tuberosum L.) and sweet potatoes Ipomoea batatas (L.) Lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESl-MS/MS). Mol. Nutr. Food Res., 50,1201-1211. [Pg.118]

A quantitative HPLC method for the analysis of sphingolipids as their perbenzoyl derivatives was first developed for ceramides (5). Ceramides can be conveniently derivatized with benzoic anhydride in pyridine (3 hrs at 110°C) and the products formed have been utilized for the quantitative analysis of NFA and HFA ceramides in normal and Farber s disease tissue. Iwamori and Moser also utilized this procedure for the analysis of ceramides in Farber s disease urine (6). More recently Iwamori and Moser (7) established that the ceramide derivatives formed by reaction with benzoyl chloride or benzoic anhydride are analogous to those formed with cerebrosides. They also characterized the behavior... [Pg.3]

H. Egge Structural analysis of blood group ABH, I, i, Lewis and related glyco-sphingolipids. Application of high resolution NMR and FAB mass spectrometry... [Pg.54]

Byrdwell, Y.C. Dual parallel mass spectrometers for analysis of sphingolipid, glycerophosphohpid and plasma-logen molecular species. Rapid Commun. Mass Spectrom. 1998, 12, 256-272. [Pg.937]

In lower organisms the structural analysis was reported of a new glyco-sphingolipid (GSL) from the LPS-lacking Sphingomonas adhaesiva (whilst in... [Pg.344]

Bielawski, J., Szulc, Z. M., Hannun, Y. A., Bielawska, A. Simultaneous quantitative analysis of bioactive sphingolipids by high-performance liquid chromatography-tandem mass spectrometry. Methods 39 (2006) 82-91. [Pg.287]

Merrill AH, Jr., Sullards MC, Allegood JC, Kelly S, Wang E. Sphingolipidomics high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometry. Methods 36(2005) 207-224. [Pg.383]

Fig. 13. Major fragmentation sites of sphingolipids that are useful for analysis by electrospray tandem mass spectrometry [6]. Fig. 13. Major fragmentation sites of sphingolipids that are useful for analysis by electrospray tandem mass spectrometry [6].
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