Spermatocyte Test on Treated Males

TABLE 3. Minimum and Maximum Incidence per Animal of Spermatocytes with Translocation Configurations after Spermatogonial Irradiation 

Reference X-ray dose (r) Spermatocytes with translocation configurations Minimum (%) Maximum (%)  

Types of aberrations other than translocations are much less frequent. Small fragments are sometimes observed in dividing spermatocytes of mice given X-irradiation, but their incidence is always very low. 46-48) The mean number of X-Y univalents remains unaltered in most animals, although a few individuals can display an increase to as much as 40%. The causes and the meaning of this high incidence are so far unknown, and no relation seems to exist between the type of treatment and the number of animals with a high rate of X-Y univalents. Finally, the incidence of polyploidy seems not to increase after irradiation. 

---

The characteristic meiotic configurations of spermatocyte I chromosomes at the diakinesis first metaphase stage thus provide a reliable technique for the detection of rearrangements or other chromosome aberrations induced in spermatogonia of the treated males spermatocyte test on treated males). Cytological analysis of dividing spermatocytes can also be used to confirm in Fi males the presence of translocations indicated by semisterility, the Fi females being indirectly tested by examination of sons F translocation test) (Table 1). 

Often, the time interval between treatment and observation chosen for the spermatocyte test on treated males W2is too short. Thus many authors were able only to score some unstable aberrations in spermatogonia or to detect the anomalies induced in spermatocytes. Furthermore, some of the anomalies claimed to be the result of the treatment might even be considered a normal process under the conditions employed. Apparently contradictory results have even been sometimes obtained in different experiments with the same chemical, since the rate of trans-... 

Chemical Spermatocyte test on treated males Reference Fi translocation test Reference... 

The spermatocyte test on male mice given caffeine for a long period in their drinking water ( > 6) or treated with a single dose of 0.2 g/kg or with daily doses of 0.25 g/kg for 3 weeks gave negative results. c 6)... 

---