Spectra uridine

The UV spectra of azacytidine are similar to those of uridine and 6-azauridine but differ from those of cytosine and cytidine. On the other hand, the spectrum of the 4-dimethylamino derivative is different from that of 6-azacytidine and similar to that of cytidine. "... 

Comparison of the spectrum of uridine with data of 4-thiouridine, 2,4-dithiouridine, thymidine, 4-thiothymidine, cytidine and 2-thiocytidine allows the assignment of all carbon resonances of these pyrimidine moieties. Further confirmation of these assignments is obtained from the proton off-resonance decoupled 13C-spectra of these nucleosides, as has been demonstrated for thymidine The pyrimidine CH3 and CH resonances of the proton broadband-decoupled spectrum are split into a quartet and a doublet, respectively, in the proton off-resonance spectrum, and can thus be easily assigned. 

Fig. 6.—Ultraviolet Absorption Spectrum of Uridine at pH Values Indicated. (Adapted from Fox and Shugar.87)...

A suspension of 4.00g (6.75 mmol) of 3, 5 -bis-0-(p-nitrobenzoyl)-2 -deoxy-5-(trifluoromethyl)uridine in 250 ml of methanol was treated with 10 ml of diisopropylamine and refluxed until it had dissolved (about 18 minutes), and the solution was concentrated. The dry residue was partitioned between 50 ml of chloroform and 50 ml of water. The chloroform layer was washed with 20 ml of water, and the combined aqueous layers were concentrated. A low ultraviolet extinction (e 7200 and 262 pm pH 1) and the presence of isopropyl signals in the NMR spectrum (two singlets aty8.73 and 8.85) indicated the dry residue contained diisopropylamine, probably as a salt with the relatively acidic heterocyclic N-H in 14. 

The proton signals of uridine (36) dissolved in deuterium oxide are well separated at 220 MHz, and a complete analysis and simulation of the spectrum by means of the LAOCOON II computer program (see Section V, p. 74) has been reported.107 It was concluded from the temperature-independence of the coupling constants and chemical shifts of the D-ribofuranoid moieties of uridine, /3-pseudouridine (40), and a-pseudouridine (41), that each of these compounds... 

SAC-CI method was applied to calculate the electronic CD spectrum of uridine [43], Based on theoretical CD and absorption spectra, observed peaks in the experimental spectra were assigned. The rotational strength (R) in the length form [44] was calculated as imaginary part of the inner product of the electric transition dipole moment (ETDM) and magnetic transition dipole moment (MTDM). 

Figure 4-2. (a) Absorption and (b) CD spectra of uridine. In the theoretical CD spectrum, the calculated rotational strengths (solid vertical lines) were convoluted with die Gaussian envelopes... 

Uridine in DMSO-d6 NOESY spectrum 500 msec mixing time... 

Figure 14.42. Downfield region of the temperature-dependent H spectrum of a 1 1 mixture of adenosine and poly U. The Hg and H2 resonances of adenosine and the H6 resonance of uridine are visible in this region. (From ref. 6. Reprinted with permission of S. I. Chan.)...

A few attempts have been made to observe the n tt transitions in uracils. No Cotton effect derived from an n tt transition is observed in the CD spectrum of uridine. Such transitions are observed in some 6-azauracil nucleosides, but they are located at the long-wavelength side of the first band (Ban). On the other hand, Eaton and... 

In the original WATERGATE-1 experiment, using selective inversion element (a), a net rotation of zero for the solvent magnetization is achieved while the 180° pulse inverts all the other coherences. In Check it 52.3.5 the efficiency of the WATERGATE-1 sequence is demonstrated for the IH spectrum of uridine (1%), a nucleoside of uracil and ribose, in water (99%). 

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