Spectra bacteriophage

Figure 5-53 (A) JH NMR spectrum of a 17 base-pair DNA segment from the operator sequence OR3 from bacteriophage X in D20 at 37°C. (B) Combined COSY above the diagonal and NOESY (below the diagonal) spectra. C5H and C6H J coupling is established from cross-peaks in box d for cytosines and in box a for thymines. Two unresolved cross-peaks give rise to the more intense spots marked by arrows. Box b contains cross-peaks from scalar coupling of the two H2 protons to the HT protons of the deoxyribose rings. Most of the aromatic proton resonances could be assigned using the NOE cross-peaks in box f. For further details see Wemmer et al.676 See also Bax and Lerner.672 Courtesy of B. Reid.

Fig. 12. (a) Experimental PISEMA spectrum of uniformly " N-labeled Y21M fd bacteriophage... 

Fig. 2 PSi microcavity-based detection of bacteriophage lambda (adapted from [9]). The solid line represents the photoluminescence spectrum of the DNA-derivatized device, while the dotted line shows the photoluminescence spectrum of the sensor following exposure to the bacteriophage virus...

JPM van Duynhoven, PJM Folkers, CWJM Prinse, BJM Harmsen, RNH Konings, CW Hilbers. Assignment of the I I NMR spectrum and secondary structure elucidation of the single-stranded DNA binding protein encoded by the filamentous bacteriophage IKe. Biochemistry 31 1254-1262, 1992. 

NMR studies of the motions of the aromatic amino acids of the fd bacteriophage protein were made, and the results are shown in Fig. 8.12. The experimental spectrum fits the calculated flip-averaged spectrum better than it fits the rotationally averaged spectrum. Therefore the rings are executing 180 flips in less than 1 ns, and probably in less than 0.01 ns. Additionally, the experimental spectrum is narrower than expected from the static chemical-shift tensor, and so additional molecular motion of the main chain is also involved. 

Akutsu et al. (1980) have reported P-NMR studies of lipid-containing viruses (e.g., bacteriophage PM2) that are spherical in shape with a hydrated diameter of 600 A and possessing a lipid bilayer. The virus contains only four proteins, namely, proteins I, II, III, and IV. The representative P-NMR spectra are shown in Fig. 14, where a 60% sucrose solvent was used to eliminate the influence of the overall rotational motion of the virus on the spectrum. It is clear firom the spectra A and C that there are two major components an axial symmetric powder pattern superimposed on the broad component. Akutsu and co-workers assigned these two components to a liquid-crystalline bilayer and the DNA inside the virus. Simulated spectrum B b d on the spectra of extracted lipids and T4 phage, which is known to have no lipid membrane, is in good agreement with the observed spectrum A or C. In the presence of 4-6 Af urea, the nucleocapsid was... 

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