Spectinomycin, detection

Potassium permanganate provides a method for the detection of sugars and sugarlike molecules, and drugs with aliphatic double bonds. It is used in TLC identity checks for the antibacterial agents clindamycin and lincomycin and in a check for related substances in spectinomycin. 

Residue depletion studies with piglets orally dosed with 30 mg spectinomycin/kg bw/day for 5 consecutive days showed that the average concentrations of the parent drug in liver, kidney, muscle, and skin/fat were 1030, 7700, less than 300, and less than 394 ppb, respectively, at 3 days, and less than 198, 500, 300, and 26 ppb, respectively, at 14 days postdosing. Broilers given water dosed at 50 mg spectinomycin/kg bw/day for 5 consecutive days were not found to contain detectable spectinomycin concentrations (limit of detection 500 ppb for liver and kidney and 250 ppb for muscle and skin/fat) in any edible tissue even only 1 day after the last dose. 

PG Schermerhom, PS Chu, PJ Kijak. Determination of spectinomycin residues in bovine milk using liquid-chromatography with electrochemical detection. J Agric Food Chem 43 2122-2125, 1995. 

Medina, M.B. 2004. Development of a fluorescent latex immunoassay for detection of a spectinomycin antibiotic. J. Agric. Food Chem. 52 3231-3236. 

Walker, J.B., 1995, Enzymatic synthesis of aminocyclitol moieties of aminoglycoside antibiotics from inositol by Streptomyces spp. Detection of glutamine-aminocyclitol aminotransferase and diaminocyclitol aminotransferase activities in a spectinomycin producer. J. Bacteriol. 177 818-822. 

Clark NC, Olsvik O, Swenson JM, Spiegel CA, Tenover FC. Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis. Antimicrob Agents Chemother 1999 43(l) 157-60. 

The aminoglycoside antibiotic spectinomycin was assayed in plasma by formation of its 2,4-dinitrophenyl hydrazone, reversed-phase chromatography and detection at 405 nm, while neomycin in plasma and urine was assayed by ion-pair reversed-phase chromatography with post-column derivatization with o-phthalaldehyde and fluorescence detection. The chemotherapeutic agent morpholino-anthracycline MX2 (4), its metabolites (5) and (6), and the deglycosylated anthracycline were determined in biological samples by reversed-phase h.p.l.c. ... 

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