Soybean lipoxygenase-1 structure

Segraves, E.N., Chruszcz, M., Neidig, M.L., Ruddat, V., Zhou, J., Wecksler, A.T., Minor, W., Solomon, E.I., Holman, T.R. 2006. Kinetic, spectroscopic, and structural investigations of the soybean lipoxygenase-1 first-coordination sphere mutant, Asn694Gly. Biochemistry 45 10233-10242. 

Earlier, carboxylate oxygens were proposed to be ligands in soybean lipoxygenase-1 that was analysed by extended X-ray absorption fine structure [99]. Later, the crystallographic data showed that COO" of the C-terminal isoleucine was another ligand [65,80]. The C-terminal isoleucine residue is well-conserved in... 

Although soybean lipoxygenase-1 was already crystallized in 1947 [22] it was not until 1990 that the first lipoxygenase-1 and -2 crystals suitable for X-ray crystallography were obtained [154-156]. The first crystal structure, at 2.6 A resolution, of soybean lipoxygenase-1 was reported in 1993 [157,158]. Recently, the lipoxygenase-1 structure with a 1.4 A resolution, and the crystal structure of soybean lipoxy-genase-3 with a 2.6 A resolution have been reported [62,159]. 

Figure 26. Structures of soybean lipoxygenase 1, based on chrystallographic determination (A), by Minor etal [307, 308] (B), by Boyington etal [310].

Minor W, Steczko J, Stec B, Otwinowski Z, Bolin JT, Walter R, Axehod B. 1996. Crystal structure of soybean lipoxygenase L-1 at 1.4 A resolution. Biochemistry 35(33) 10687-10701. 

Formation. Although bicyclo[2.2.l]endoperoxides are important intermediates in prostaglandin chemistry, they have been synthetically elusive. Autoxidation of polyunsaturated fatty. acids does lead to mixtures of compounds containing the endoper-oxide structure (, 21). We (22) were also able to isolate prostaglandin products from the sequence described below. The lipid hydroperoxide (prepared from soybean lipoxygenase and the fatty acid (23) was treated with a free radical initiator and the products, following borohydride reduction, were compared to authentic prostaglandins (24). 

Tomchick, D.R. Phan, P Cymborovski, M. Minor, W. Holm, T.R. Structural and Functional Characterization of Second-Coordination Sphere Mutants of Soybean Lipoxygenase-1. Biochemistry 2001, 40, 7509-7517. 

Shibata D, Steczko J, Dixon JE. Hermodson M, Yazdanparast R. Axelrod B. Primary structure of soybean lipoxygenase-1. J Biol Chem 1987 262 10080-10085. 

Different isoforms of 15-lipoxygenase have been purified to homogeneity from mammalian sources, including reticulocytes and leukocytes, and complementary DNAs encoding them have been cloned. The reticulocyte 15-lipoxygenase is a cytosolic protein, whose amino acid sequence is 65% similar to human 5-lipoxygenase and 45% similar to type I soybean lipoxygenase (whose tridimensional structure has been resolved by... 

Srinivasulu, S. and Rao, A.G.A. 1993. Kinetic and structural studies on the interaction of surfactants with lipoxygenase LI from soybeans (Glycine max ). J. Agric. Food Chem. 41 366-371. 

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