Structure of soybean lipoxygenase

Figure 26. Structures of soybean lipoxygenase 1, based on chrystallographic determination (A), by Minor etal [307, 308] (B), by Boyington etal [310].

Minor W, Steczko J, Stec B, Otwinowski Z, Bolin JT, Walter R, Axehod B. 1996. Crystal structure of soybean lipoxygenase L-1 at 1.4 A resolution. Biochemistry 35(33) 10687-10701. 

Shibata D, Steczko J, Dixon JE. Hermodson M, Yazdanparast R. Axelrod B. Primary structure of soybean lipoxygenase-1. J Biol Chem 1987 262 10080-10085. 

Although soybean lipoxygenase-1 was already crystallized in 1947 [22] it was not until 1990 that the first lipoxygenase-1 and -2 crystals suitable for X-ray crystallography were obtained [154-156]. The first crystal structure, at 2.6 A resolution, of soybean lipoxygenase-1 was reported in 1993 [157,158]. Recently, the lipoxygenase-1 structure with a 1.4 A resolution, and the crystal structure of soybean lipoxy-genase-3 with a 2.6 A resolution have been reported [62,159]. 

Tomchick, D.R. Phan, P Cymborovski, M. Minor, W. Holm, T.R. Structural and Functional Characterization of Second-Coordination Sphere Mutants of Soybean Lipoxygenase-1. Biochemistry 2001, 40, 7509-7517. 

Segraves, E.N., Chruszcz, M., Neidig, M.L., Ruddat, V., Zhou, J., Wecksler, A.T., Minor, W., Solomon, E.I., Holman, T.R. 2006. Kinetic, spectroscopic, and structural investigations of the soybean lipoxygenase-1 first-coordination sphere mutant, Asn694Gly. Biochemistry 45 10233-10242. 

Earlier, carboxylate oxygens were proposed to be ligands in soybean lipoxygenase-1 that was analysed by extended X-ray absorption fine structure [99]. Later, the crystallographic data showed that COO" of the C-terminal isoleucine was another ligand [65,80]. The C-terminal isoleucine residue is well-conserved in... 

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