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Sortase-mediated protein ligation

Keywords Click ligation Diels-Alder ligation Expressed protein Ugation GPI anchor MIC ligation Peptide synthesis Protein lipidation Rab Ras Rheb Sortase-mediated protein ligation... [Pg.137]

Sortase- mediated protein ligation Protein with a C-terminal LPXTG motif Native peptide bond with additional amino acid sequence K-Ras4B, lipidated GFP, GPI modified GFP... [Pg.156]

Recently, Guo and co-workers reported sortase-mediated chemoenzymatic synthesis of a GPl-anchored protein [100]. The GPI anchor 84 featuring the common glycan core, a lipid and an additional double glycine unit was coupled to the model protein GFP 83 by SrtA (Scheme 15d). This work has demonstrated that SrtA could accept a complex GPI anchor, suggesting that SrtA-mediated protein ligation is a versatile approach for protein synthesis. [Pg.165]

Scheme 15 Site-specific lipid attachment through sortase-mediated transpeptidation. (a) Mechanism of sortase-mediated ligation, (b) Semisynthesis of lipid modified GFP protein by sortase-mediated ligation, (c) Semisynthesis of lipidated K-Ras4B protein, (d) Semisynthesis of GPI modified GFP protein... Scheme 15 Site-specific lipid attachment through sortase-mediated transpeptidation. (a) Mechanism of sortase-mediated ligation, (b) Semisynthesis of lipid modified GFP protein by sortase-mediated ligation, (c) Semisynthesis of lipidated K-Ras4B protein, (d) Semisynthesis of GPI modified GFP protein...

See other pages where Sortase-mediated protein ligation is mentioned: [Pg.47]    [Pg.351]    [Pg.351]    [Pg.351]    [Pg.138]    [Pg.155]    [Pg.157]    [Pg.164]    [Pg.69]    [Pg.295]    [Pg.47]    [Pg.351]    [Pg.351]    [Pg.351]    [Pg.138]    [Pg.155]    [Pg.157]    [Pg.164]    [Pg.69]    [Pg.295]    [Pg.146]    [Pg.351]    [Pg.164]    [Pg.479]   
See also in sourсe #XX -- [ Pg.137 , Pg.146 , Pg.164 ]




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