Some recurrent problems in the hybridoma technique

HAT solutions should be tested with myeloma cells (should not survive). Failing HAT solutions produce deleterious overgrowth by myeloma cells. On the other hand, no growth at all could be indicative 

Infections should not occur with good practices (vol. 8 in this series by Adams, 1980). The most common problem is caused by fungi, particularly if incubators with built-in fans have been contaminated. The use of antibiotics is widespread but could lead to antibiotic-resistant mycoplasmas (Rahman et al., 1967) or chromosome damage (Leonard and Botis, 1975). 

Electron microscopy is a reliable way to detect mycoplasmas (Brown et al., 1974), but the mycoplasma stain (Hoechst 33258 Chen, 1976) is convenient. 

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