Solvent degasser

Thermo SeparaAons Products HPLC system with an autosampler, solvent degasser and column heater interfaced to aFinnigan MAT 7000 mass spectrometer or equivalent insAumentation opAmized for the detection of azinphos-methyl. 

Solvent A water containing 0.1% (v/v) formic acid Solvent B acetonitrile containing 0.1% (v/v) formic acid A gradient is run from 90% A to 10% A over 12 min. The solvent composition is taken back to 90% A at 12.5 min and equilibrated until 17.5 min (solvents degassed)... 

Solvent degassing (LC) 553 Solvent demixing (TLC) 660 Solvent effects, splitless injection (GC) 250 Solvent extraction 753 applications 766 hoBogenizer 761 i plitgers 756 micTMethods 764 microwave 761 optimization 753 shake flask 761 solvent reduction 763 Soxhlet 762 Solvent (LC)... 

Figure 5.2 A, valve, column and flow cell asseidily of a ainiaturized liquid chromatograph for use with small bore columns (Reproduced with permission from ref. 14) and B, mobile phase reservoir designed for solvent degassing by heat and helium sparging (Reproduced with permission from ref. 34. Copyright Elsevier Scientific Publishing Co.)...

The solvent reservoir is a storage container made of a saterial resistant to chemical attack by the mobile diase. In its simplest fora a glass jug, solvent bottle or Erlenmeyer flask with a cap and a flexible hose connection to the pump is adequate. The PTFE connecting hose is terminated on the solvent side with a 2 micrometer pore size filter to prevent suspended particle matter from reaching the pump. In more sophisticated instruments the solvent reservoir may also be Cjquipped for solvent degassing, as... 

Quenching Impurities in the mobile phase, particularly oxygen, may entirely quench the signal from low concentrations of fluorescent compounds (see solvent degassing). 

Model RR/066 351 and 352 pumps models 750/16 variable-wavelength UV monitor detector 750/11 variable filter UV detector, MPD 880S multiwave plasma detector, 750/14 mass detector 750/350/06 electrochemical detector refractive index detector HPLC columns column heaters, autosamplers, pre-columns derivatization systems, solvent degassers, preparative HPLC systems... 

Solvent degassing is needed because gas bubbles in the pump head can cause malfunctions (air-lock) or flow errors (low flow). Dissolved air often... 

FIGURE 8 A schematic of a 4-solvent on-line vacuum degasser. The preferred device for solvent degassing. 

Helium Sparging—A solvent degassing technique in which helium gas is bubbled through solvents to displace dissolved gases before solvent mixing, compression, and pumping. 

Solvent degassing is important to ensure that air bubbles do not outgas and interfere with accurate delivery of flow. Solvents can be degassed by... 

Glasses. The solvents used to form the glass with polymers should be clear in the UV region to allow maximum absorption of radiation by the polymer. The ESR of irradiated solvents should not interfere with that of the polymer. Three solvents were found to be close to ideal tetrahydrofuran (THF) p-dioxane (DX) and tetrahydropyran (TP). All three were purified by repeated distillation and column chromatography. Poly(vinyl chloride) and the copolymer solutions (5-15%, w/v) were prepared from these solvents, degassed, sealed under vacuum (10-6 torr), and irradiated. 

These include solvent reservoirs and inlet filters, solvent degassing facilities and one or more pumps with associated pressure and flow controls. Most systems are microprocessor or computer controlled enabl.ing parameters to... 

Solvent degassing and backpressure regulators after the detector minimize bubble formation. It is a helpful practice to perform blank runs every day to provide reference data that can be consulted at a later date. 

Replace the lamp even if the energy at e.g. 254 nm is still acceptable. Replace the flow cell windows. Check if the mobile phase is UV transparent and clean. Is the solvent degassed properly Use a degasser. 

HPLC ACCESSORIES AND DATA HANDLING SYSTEMS 4.9.1 Solvent Degasser... 

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