Solutions and media used for the cloning procedures

Many of the recipes and details are given in Experiments in Molecular Genetics by Jeffrey H. Miller (1972) which is also a valuable source of descriptive detail for many of the bacteriological techniques used  

Sucrose solution 0.25 M Tris-EDTA Pancreatic RNAase solution 

25 M Tris-HCl (pH 8.0), 0.25 M-EDTA Dissolve pancreatic RNAase in Low Tris buffer to give a concentration of lOmg/ml. Boil for 2 min. and store at —20°C. 

Dissolve in H O to give a concentration of 10 mg/ml. Handle only with gloves. 

2% solution of 5-chloro-4-bromo-3-in-dolyl-/3-D-galactoside in dimethyl-for-mamide. Protect from light and store at 

---