Solubilization of the membrane receptor

As indicated earlier, the intent of this section was not to be global with respect to the scope of its coverage, but rather to discuss in general terms some considerations common to the study of ligands which interact with membrane receptors and, thereby, elicit post-binding events. Many of the examples chosen have been drawn from my experience with the follitropin-gonadal receptor system, but they provide instances of problems, concerns and caveats in use of techniques and interpretation of results that are common to this particular field of study. The reader is referred to the specific examples of hormone receptor interactions to follow, wherein aspects of the problems not germaine to this section, such as, for example, techniques for purification of solubilized receptors, are considered in detail. 

Cuatrecasas, P. (1974) In Annual Reviews of Biochemistry (Snell, E.E., ed.) pp. 169-214. Annual Reviews, Inc., Palo Alto, California. 

ASHA SINGH KHANNA and DAVID MORTON WAISMAN 

Cell Regulation Research Group, Department of Medical Biochemistry, The University of 

This chapter deals with various aspects of metabolism of protein hormones, in particular, how these regulatory molecules are synthesized and stored as precursor molecules and proteolytically processed to generate biologically active hormones. The mechanisms involved in secretion and degradation are also described. 

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