Solid phase, in chromatography

Ferrer and Barcelo [131] used online solid-phase-liquid chromatography mass spectrometry for the simultanous determination of organotin antifouling herbicides in marine water. The solid-phase extraction was carried out on polymeric cartridges after percolation of 100 ml of the seawater sample, and... 

Thin layer chromatography was carried out on 20x20cm glass plates coated 0.25mm thick with a suitable support and dried overnight. Silica gel G, silica gel H and cellulose were examined as the solid phases for chromatography of methanearsonate, arsenite and arsenate. Several sprays for the visualization of the arsenicals on plates were tested. Three of the more successful reagents and the colour produced with final product are shown in Table 13.2. 

Stationary phase in chromatography, the porous solid or hquid phase through which an introduced sample passes. The different affinities the stationary phase has for a sample allow the components in the sample to be separated, or resolved. 

The use of hydrophobic stationary phases and aqueous mobile phases in chromatography on so-called reversed phase (RP) materials is widely established. Reversed phase materials typically consist of alkyl chains (mainly C18 in length) immobilised on silica material. The chromatographic retention of a solute on such a system directly depends on its partition between the aqueous mobile and the hydrophobic solid phase (Berthod et al. 2004). Therefore lipophilicity (expressed as partition coefficient logP between stationary and mobile phase, can be estimated from the retention behaviour of the solute of interest on reversed phase material as reviewed by Nasal et al. (2003). 

Although in a number of cases chromatography is used in packed-bed mode, there are an increasing number of examples of the use of solid phases in fluidized-bed mode. This does not change the adsorption phenomenon based on the complementarity of the solute for the solid phase. Fluidized-bed columns are essentially used to resolve specific problems related to the feedstock, as detailed later. 

R. P. Pioch, U. S. Patent 2,997,470 (1961). iA. Stoll and A. Hofmann, Helv. Chim. Acta, 38,421 (1955). Showed two components on tic which were not separable by solid-phase column chromatography. We believe that the two components represent diastereoisomers differing at the asymmetric carbon in R2. Concentration 0.8 in pyridine at 26°. "Concentration 0.5 in pyridine. 

Over the past twenty years, fluids above their critical temperatures and pressures have been used for a variety of analytical purposes first as mobile phases in chromatography [1,2], thus giving rise to supercritical fluid chromatography, and then as solvents for the selective extraction of species — from solid samples in most cases [3-5]. 

Mobile phase In chromatography, a liquid or a gas that carries analytes through a liquid or solid stationary phase. 

Stationary phase In chromatography, a solid or an immobilized liquid on which analyte species are partitioned during passage of a mobile phase. 

Ion-pair distribution in liquid chromatography was initially used in systems where the counter-ion in an aqueous solution was loaded onto a solid phase. In early applications, diatomaceous earth [36] or porous ethanolyzed cellulose [37] was used as support, but the availability... 

The solid phase in HPLC columns used for organic monomers is usually some form of silica gel. "Normal" HPLC refers to chromatography using a solid phase (usually silica gel) that is more polar than the liquid phase, or solvent, so that the less polar compounds elute more rapidly. Typical solvents include ethyl acetate, hexane, acetone, low molecular weight alcohols, chloroform, and acetonitrile. For extremely polar compounds, such as amino... 

Stationary phase In chromatography, the liquid or solid phase in the chromatographic column upon which analyte species are separated. 

Reversed-phase high performance liquid chromatography (RP-HPLC)—an HPLC technique where the mobile phase is more polar than the solid phase. In RP-HPLC compounds are separated based on their hydrophobic character. 

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