Solid-phase extraction grape seeds

Zhu, Y., Coury, L.A., Long, H. et al. (2000) Liquid chromatography with multichannel electrochemical detection for the determination of resveratrol in wine, grape juice, and grape seed capsules with automated solid phase extraction. J. Liq. Chromatogr. Related TechnoL, 23, 1555-1564. 

In a companion study, M. Palma et al. studied the extraction of grape seeds with pure SF CO2 and analyzed the derivatized extracts by GC-MS. These extracts were found to contain volatiles such as aliphatic aldehydes in addition to fatty acids and sterols. Even though we used similar conditions for our SF extraction and GC-MS analysis, we were unable to detect any similar volatile compounds. To further investigate the presence of volatiles in the cranberry seed extract, we adapted a solid phase microextraction (SPME) method from the work of Jelen et al. who had earlier developed it for the characterization of volatile compounds in different vegetable oils. SPME followed by gas chromatog[raphy was performed on the headspace of the cranberry seed extract to test for the presence of volatile compounds. The GC trace failed to show the elution of any components for either the SF or Soxhlet extract. 

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