Sodium dodecyl sulfate gel electrophoresis

Guttman, A, On the Separation Mechanism of Capillary Sodium Dodecyl Sulfate-Gel Electrophoresis of Proteins, Electrophoresis 16, 611, 1995. 

Guttman, A., Shieh, P, Lindahl, J., and Cooke, N., Capillary sodium dodecyl sulfate gel electrophoresis of proteins. II. On the Ferguson method of polyethylene oxide gels, /. Chromatogr., 676, 227, 1994. 

Guttman, A. and Nolan, J., Comparison of the separation of proteins by sodium dodecyl sulfate-slab gel electrophoresis and capillary sodium dodecyl sulfate-gel electrophoresis, Anal. Biochem., 221, 285, 1994. 

A. Gerstner, Z. Csapo, M. Sasvari-Szekely, and A. Guttman, Ultrathin sodium dodecyl sulfate gel electrophoresis of proteins Effect of gel composition and temperature on the separation of sodium dodecyl sulfate-protein complexes, Electrophoresis, 21, 834 (2000). 

Hu, D. H., Kimura, S., and Maruyama, K. (1986). Sodium dodecyl sulfate gel electrophoresis studies of connectin-like high molecular weight proteins of various types of vertebrate and invertebrate muscles./. Biochem. (Tokyo) 99, 1485—1492. 

Creatine kinase was purified from rabbit muscle by the method of Kuby et al, (4). Rabbit muscle pyruvate kinase was purchased from Boehringer. Porcine muscle adenylate kinase was purchased from Sigma, and was further purified by gel filtration on Sephadex G-50. The enzymes were homogeneous as judged by their specific activities and by their migration as single components in sodium dodecyl sulfate gel electrophoresis. Proton NMR spectra at 250 MHz of 0.5-2.0 mM enzyme sites in 0 solution were obtained with a Bruker WM 250 MHz pulse FT spectrometer at 25°. At least 256 transients were accumulated over 8192 data points using 16 bit A/D conversion. Relaxation rates and histidine pK values were determined by standard NMR methods (5, 6),... 

Li, Y., Buch, J.S., Rosenberger, F., DeVoe, D.L., Lee, C.S., Integration of isoelectric focusing with parallel sodium dodecyl sulfate gel electrophoresis for multidimensional protein separations in a plastic microfluidic network. Anal. Chem. 2004, 76, 742-748. 

Improved silver staining procedure for fast staining in PhastSystem Development Unit. 1. Staining of sodium dodecyl sulfate gels. Electrophoresis 9,... 

Guttman A (1996). Capillary sodium dodecyl sulfate-gel electrophoresis of proteins. Electrophoresis. 17 1333-1341. 

Sotelo, C.G., Pineiro, C., Perez-Martin, R.I., and Gallardo, J.M. 2000. Analysis of fish and squid myofibrillar proteins by capillary sodium dodecyl sulfate gel electrophoresis Actin and myosin quantification. Ear. Food Res. Technol. 211, 443-448. 

Maruyama, K., et al. (1984). Detection of Calcium Binding Proteins by Ca Autoradiography on Nitrocellulose Membrane After Sodium Dodecyl Sulfate Gel Electrophoresis, J. Biochem. 95 511-519. 

Maruyama, K., Mikawa, T., and Ebashi, S. (1984) Detection of calcium binding proteins by Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis./. Biochem. 95, 511-519. 

Generally, purification is measured by determining the enzyme specific gravity using a spectrophotometer, sodium dodecyl sulfate gel electrophoresis, isoelectric focusing, and a mass spectrophotometer. 

Blue staining profile of membrane proteins separated by sodium dodecyl sulfate gel electrophoresis. 

Pre-cast 4-20 % gradient gels for sodium dodecyl sulfate gel electrophoresis (SDS-PAGE) (e.g., Tris-glycine gels). 

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