Sodium dihydrogen phosphate monohydrate

O-phosphoric acid and/ or sodium hydroxide may be used for pH adjustment dihydrate 0.45mg/mL sodium dihydrogen phosphate monohydrate 0.1 mg/mL methionine 0.1 mg/mL Poloxamer 188 ... 

Methylene blue reagent 30 mg methylene blue in 500 mL water add 41 mL of 6 A H2S04 and 50 g sodium dihydrogen phosphate monohydrate (NaH2P04 H20) dilute to 1 L. 

The Step 5 product (42.11 mmol) was added to 198 ml THF/r-butyl alcohol/2-methyl-2-butene, 1 1 1.3, cooled to 0°C, then treated with sodium chlorite (331.1 mmol), and sodium dihydrogen phosphate monohydrate (256.7 mmol) dissolved in 102 ml water. The biphasic solution was stirred for 10 minutes at 0°C and then 1 hour at ambient temperature and was then concentrated. The residue was diluted with 200 ml water and the pH adjusted to 3 using saturated NaHC03 solution and 1M HC1. The aqueous solution was extracted four times with THF/CH2C12, 1 2, dried with MgS04, and concentrated. The residue was purified by trituration with diethyl ether and the product isolated in 88% yield as a white solid. 

The buffer was prepared by dissolving 100 g of sodium dihydrogen phosphate monohydrate (Alfa Johnson Matthey) into 200 mL of glass-distilled water. The pH of the solution was adjusted to 6.9 with the addition of concentrated sodium hydroxide solution (MC8 Reagent) and verified with a pH meter recently calibrated between 4-7 pH units using Fisher standards. The buffer solution was diluted to a final volume of 500 mL (i. 45 M) and stored in a clean plastic bottle. 

Sodium dihydrogen phosphate, monohydrate (Sodium phosphate, monohasic), NaH2P04-HjO. Mol. wt. 137.99. Suppliers Baker, Fisher. 

NH5C03 ammonium hydrogen carbonate 1066-33-7 25.00 1 5860 1 2882 NaH405P sodium dihydrogen phosphate monohydrate 10049-21-5 25.00 2.0400 1... 

Sodium dihydrogen phosphate monohydrate Buffering agent ic... 

Sodium dihydrogen phosphate monohydrate Sodium dihydrogen phosphate dihydrate PhEur Natrii dihydrogenophosphas dihydricus... 

Sodium dihydrogen phosphate monohydrate Na04P04-040 10049-21-6 137.993 wh hyg cry 100 dec 94.9 iEtOH... 

IMP (> 98 %, Sigma, USA), polyethylene glycol f-octylphenyl ether, TX-lOO ( 99 %, Fluka, Switzerland), and KCl (> 99.9 %, Ranbaxy, India) were used as received. Doubly distilled and deionized water (sp. cond. = 1-2 pS-cm-i) was used as the solvent. Tiisodium phosphate dodecahydrate (TSP), and sodium dihydrogen phosphate monohydrate (SDP) were of reagent grades obtained from Merck. 10 mM Sodium phosphate (SP) buffer solutions were used throughout as solvent. The pH of the IMP solutions was measured with an ELICO pH meter (model LI 120) using combined electrode. 

Phosphate-buffered saline (PBS) To make 1 liter, dissolve 6.9 g of sodium dihydrogen phosphate monohydrate (NaH2P04-H20) and 9 g sodium chloride (NaCl) in distilled water, adjust pH to 7.2, and bring to a total volume of 1 liter. 

Mobile phase MeCN MeOH buffer 5 2.5 92.5 (Prepare mobile phase by dissolving 30 mg sodium 1-heptanesulfonate hydrate, 1.379 g sodium dihydrogen phosphate monohydrate, 2.5 mL MeOH, and 5 mL MeCN in 100 mL water. Adjust pH to 3.0 with 100 mM phosphoric acid.)... 

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