Sodium borohydride, hydroperoxide determination

SOD see Superoxide dismutase Sodium, zeolite Na-Y photooxygenation, 870-4, 885-6, 887 Sodium borohydride, hydroperoxide determination, 687, 690, 691, 692 Sodium hypochlorite, aUyhc hydroperoxides, 673... 

For each solvent, aldehyde and hydroperoxide reductions via the A-26, IRA-400, XE-279, and IRA-458 borohydride-form resins were investigated. Amorphous sodium borohydride and tetraethylammonium borohydride were used for comparison. Studies were run at both ambient temperature and at 45°C. Percent hydride of each resin was determined immediately prior to use. 

Hydroperoxy fatty acids as such cannot be separated by GC as they decompose at high temperatures, and HPLC is probabiy the preferred method for their anaiysis [168], Nonetheiess, there are times when it is advantageous to convert them to the hydroxy derivatives by means of sodium borohydride reduction and then to the TMS ethers for GC anaiysis, for exampie for identification by GC-mass spectrometry products derived from linoleic [223,341,911,946,1005], arachidonic [124,407,568, 1005] and docosahexaenoic acids [948] have been examined in this way (the list is not intended to be comprehensive). Woollard and Mallet [1005], in particular, have presented a comprehensive list of ECL data for compounds of this type. In addition, GC methods were used for the determination of the absolute configuration of hydroperoxides formed by lipoxygenase reaction [124,568,947]. 

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