Smart screening

Engels MFM, Venkatarangan P. Smart screening Approaches to efficient HTS. Curr Opin Drug Discov Devel 2001 4 275-83. 

Engels, M. F., and Venkatarangan, P. (2001) Smart screening approaches to efficient HTS. Current Opinion Drug Discovery Development 4, 275-283. 

Engels MFM, Venkatatangam P. Smart screening approaches to efficient HTS. Curr. Opin. Drug. Disc. Dev. 2001 4 275-283. 

An x-ray area detector can be used to collect the intensities of many reflections at a time. The crystal must be oriented in many different settings with respect to the incident beam but the detector needs to be positioned at only a few positions to collect all of the data. A charge coupled device (CCD) is used as the area detector on the Siemens SMART single crystal diffractometer system. The SMART detector consists of a flat 6-cm circular phosphorescent screen that converts x-ray photons to visible light photons. The screen is coupled to a tapered fiber optics bundle which is then coupled to a one inch by one inch square CCD chip. The CCD chip has 1024 x 1024 pixels each of which stores an electrical charge proportional to the number of... 

In general, the term electrochromism is used to describe the change in light absorption as a result of an electrochemical reaction. Recent interest in electrochromism stems from its potential applications in numerous devices, such as flat screen displays, antidazzle mirrors, smart windows, and others. 

Since the development of Stage II-screening reactor systems in 1998 [71], the 48-fold reactor technology is, in the meantime, a state-of-the-art methodology in the context of robustness, cost, and efficiency, and operates 24 h a day, 7 days a week, which is eased by the use of a smart control... 

DOW in Midland, USA, performed metallocene-catalyzed polymerization of ethylene using a homebuilt tube reactor setup with advanced microflow tailored plant peripherals for heating, temperature monitoring, pressure control and dosing via smart valves and injectors. Screening of process conditions was a driver [19]. Also, flexibility with regard to temperature and pressure at low sample consumption was an issue. Quality of the information is another motivation due to the advanced process control and sensing. 

Figure 10.1 The SMART approach is built on drugs with proven in vitro efficacy, common pharmacological in vitro models, and high resolution proteomics technologies. Alternative drug targets are subsequently identified and evaluated for their potential in drug screening programs.

Table 2 Sample aliquoting tree for the supply of the vast amount of samples needed for high-throughput screening. The Liquid Master Store assembles editions of master solutions for preparation of the compound aliquots needed for multiple HTS projects. The Smart Compound Depository delivers the compound subsets from the HTS library needed for hit validation and secondary screening...

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