Small molecules separation organic monoliths

In comparison to their silica counterparts, organic polymer monoliths generally exhibit lower efficiencies in the reversed-phase HPLC separation of small molecules. This reduced performance is primarily due to the lack of mesopores (see Section 1.3.2.5) and the presence of micropores in the polymer matrix, which cause slow internal diffusion. However, there are some promising approaches trying to accomplish the chromatography of small molecules on organic polymer monoliths. 

While most of the papers deal with the separation of large molecules, a few successful separations of small molecules were recently reported."" By stacking several disks of the same chemistry into a single monolithic column, it was possible to apply it for monitoring the organic acid formation during the fermentation of yeast Yarrowia lypoli-A similar column was used for the determination... 

The molecular imprinting strategy can be applied for the recognition of different kinds of templates from small organic molecules to biomacromolecules as proteins. Some examples of separations investigated with MIP monoliths in CEC and LC are shown in Table 2. The influence of the imprinted monolithic phase preparation procedure and of the separation conditions on the selectivity and chromatographic efficiency have been widely studied [154, 157, 161, 166, 167, 192]. The performance of imprinted monoliths as chromatographic stationary phase has also been compared to that of the traditional bulk polymer packed column [149, 160]. It was shown that the monolithic phases yielded faster analyses and improved chiral separations. 

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