Slovak Academy of Sciences

Polymer Institute of the Slovak Academy of Sciences 842 36 Bratislava Slovakia... 

We would like to thank Dr. I. Lacik from Polymer Institute of the Slovak Academy of Sciences who undertook the GPC experiments, Dr. Jose Manuel Gomez-Elvira from CSIC Madrid, Spain who supplied PP of various initial molar masses, Dr. M. Pletenikova who performed some chemiluminescence measurements, Papylum project of the 5th EU community programme, Grant Agency VEGA for the grants No. 2/5109/25 and 2/6115/26 and the project KNIHA-SK. 

Polymer Institute, Slovak Academy of Sciences, Dubravskd cesta 9, 842 36 Bratislava, Slovakia. Shulamith Schlick... 

Oskar Markovic, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta, 809 33 Bratislava, Czechoslovakia (323)... 

National Technical University of Athens, Athens, Greece Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovakia Center of Excellence NANOSMART Corresponding author P. Svec, e-mail fyzisvec(g)savba.skn... 

Cellulases can also be eliminated fiom a mixture with xylanases by selective thermal inactivation. Cellulases are more thermolabile than xylanases in tiie cdlulolytic systems of the fungus Y-94 (79), T. harzianum 20), and Tkermoascus aurantiacus (77), but not in the Trickoderma reesei system (Biely, P. and Vrsanska, M., Slovak Academy of Sciences, Bratislava, unpublished results). Since cellulase thoixud inactivation causes a significant loss of xylanase also, a more convenient way to eliminate cellulase activity is by selective chemical or biological inhibition or inactivation. There appear, however, to be no reports on the existence of natural inhibitors that would be specific for cellulases. Such inhibitors of amylases and pectinases are known to occur in plants (27). 

Xylanolytic systems of yeasts and fungi can also be induced by positional isomers of xylobiose. Induction with Xylp-p-(l- 2)-Xyl/7 is analogous to the sophorose induction of cellulase in filamentous fungi (76). Xylp-p-(l- 2)-Xyl/7 and Xylp-p-(l- 3)-Xyl/7 induced xylanase in C. albidus (77), Trichosporon cutaneum (73), A. pullulans (78) and A. terreus (Hrmova, M. et al., Slovak Academy of Sciences, Bratislava, submitted for publication, 1990). In C. albidus the positional isomers behave differently than does xylobiose (77), in that the response of the cells to them was slower but the enzyme yields were higher than in the presence of xylobiose. This in-icated that isomeric xylobioses were not direct inducers. In agreement with this idea, both Xylp-p-(l- 2)-Xylp and Xylp-p-(l- 3)-Xylp were transformed to Xyl/7-p-(l- 4)-Xyl/7, the natural inducer (79). 

Institute of Chemistry, Center of Chemical Research, Slovak Academy of Sciences, 842 38 Bratislava, Czechoslovakia Institute of Experimental Physics, Slovak Academy of Sciences, 043 S3 Kosice, Czechoslovakia... 

Dr. Igor Tvaroska, Slovak Academy of Sciences, and Professor N. L. Allinger, U. Georgia, provided useful comments on the manuscript. 

Laboratory of Liquid Chromatography Polymer Institute Slovak Academy of Sciences Bratislava, Slovakia... 

DANIEL BELLUS, Polymer Institute of Slovak Academy of Sciences, Bratislava 9, Czechoslovakiaf... 

Kochetkov was awarded the Gold Medal of the Slovak Academy of Sciences in 1986 and the Haworth Memorial Medal of the UK Royal Society of Chemistry for achievements in carbohydrate chemistry in 1989. He was a member of the Chemical Society of Trance (since 1973) and a full member of the Polish Academy of Sciences (1988). 

Eva Majernikova (631), Department of Theoretical Physics, Palacky University, Tr. 17. listopadu 50, CZ-77207 Olomouc, Czech Republic and Institute of Physics, Slovak Academy of Sciences, Dubravska cesta, SK-84 228, Bratislava, Slovak Republic Nicola Manini (289), International School for Advanced Studies (SISSA), Via Beirut 4, 34014 Trieste, Italy Dip. Fisica, Universita di Milano, Via Celoria 16, 20133 Milano, Italy INFM, Unita di Milano, Milano, Italy... 

Polymer Laboratories Ltd., 243 Polymer Standards Service GmbH, 223 Precision Detectors, Inc., 109 Research Institute of Plastic, 13 Slovak Academy of Sciences, 13 Unilever Research, 151 U.S. Department of Agriculture, 141 Universite de La Rochelle, 21 Universite de Liege, 167 Universite Paris VI, 167 University of Florida, 41 University of Toronto, 265 University of Waterloo, 79 Vanderbilt University, 13... 

Acknowledgements Slovak Grant Agency VEGA (Project No. 1/0127/09) is acknowledged for financial support. This work has benefited from the Center of Excellence Program of the Slovak Academy of Sciences in Bratislava, Slovakia (COMCHEM, Contract no. II/1/2007). 

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