Size exclusion chromatography methodology

Jordan, R. C., Siler, S. F., Sehon, R. D. and Rivard, R. J., in "Size Exclusion Chromatography Methodology and Characterization of Polymers and Related Materials",... 

A. F., "Size Exclusion Chromatography Methodology and Characterization of Polymers and Related Materials", T. 

Provder,T., ed. (1984). Size exclusion chromatography Methodology and characterization of polymers and related materials. In ACS Symposium Series No. 245, American Chemical Society, Washington, D.C. 

Provder, T., Ed. Size Exclusion Chromatography Methodology and Characterization of Polymers and Related Materials ACS Symposium Series No. 245 American Chemical Society Washington, D.C., 1984. 

L. K. Kostanski, D. M. Keller, and A. E. Hamielec. Size-Exclusion Chromatography A Review of Calibration Methodologies. J. Biochem. Biophys. Meth., 58(2004) 159-186. 

Several affinity screening methodologies that include MS-based readout and work under protein-excess conditions have been developed in the past decade [1]. Some examples include affinity selection/mass spectrometry (ASMS Abbott Labs [10]), size exclusion chromatography with LC-ESI-MS (see Chapter 2 and 3 [11-19]), the use of coupled or non-coupled pulsed ultra-filtration/mass spectrometry (summarized in this chapter [11, 20-23]), restricted access phase chromatography (see Chapter 5 [24, 25]), capillary electrophoresis [26, 27], target shift mass spectrometry [28], and multitarget affinity/specificity screening (MASS, see Chapter 10 [29, 30]). 

Molecular weights are determined by end-group analysis (Mn), membrane osmometry (Mn), viscometry (Mv), size exclusion chromatography (Mm), light scattering photometry, and sedimentation (Mw). Any molar mass computed by these methods must be evaluated critically, in view of a dependence on methodology. 

Figure 7.4 Diagram illustrating the (a) general principles and (b) methodology of size-exclusion chromatography. (Sources (a) Adapted from Sheehan, Physical Biochemistry, 2000 (b) From Voet, Voet Pratt Fundamentals of Biochemistry, 2nd edn, 2006 Voet, Voet Pratt reprinted with permission of John Wiley Sons, Inc.)...

Further methodologies include dilute solution capillary viscometry, membrane osmometry, ultracentrifugation, size exclusion chromatography, and typical spectroscopic methods (e.g nuclear magnetic resonance) (Forster and Plantenberg 2002). 

---