Size exclusion chromatography conjugates

Parisi, L. and von Hunolstein, C., Determination of the molecular size distribution of Haemophilus influenzae type b-tetanus toxoid conjugate vaccines by size-exclusion chromatography, /. Chromatogr. A, 847, 209, 1999. 

To purify the conjugate from reactants that did get incorporated into the conjugate, size exclusion chromatography may be used with resins having a molecular exclusion limit able to accommodate both the labeled molecules and the final conjugate. 

The conformational mobility of a chromophoric main-chain polymer is often connected to its electronic structure. Therefore, changes in the UV-visible absorption spectra and/or chiroptical properties are spectroscopically observable as thermo-, solvato-, piezo-, or electrochromisms. It is widely reported that o-conjugating polysilanes exhibit these phenomena remarkably clearly.34 However, their structural origins were controversial until recently, since limited information was available on the correlation between the conformational properties of the main chain, electronic state, and (chir)optical characteristics. In 1996, we reported that in various polysilanes in tetrahydrofuran (THF) at 30°C, the main-chain peak intensity per silicon repeat unit, e (Si repeat unit)-1 dm3 cm-1, increases exponentially as the viscosity index, a, increases.41 Although conventional viscometric measurements often requires a wide range of low-dispersity molecular-weight polymer samples, a size exclusion chromatography (SEC) machine equipped with a viscometric detector can afford... 

It is essential that the procedure described in Section 3.1.2. be performed approx 24 h before the procedure described in Section 3.1 3, because the deprotected thiolated avidin and the maleimide derivative of the protein are unstable. Purification of the maleimide-derivatized protein by size exclusion chromatography can be performed more rapidly than dialysis, however, the former leads to dilution of the protein and a decrease in the yield of the conjugate. 

The PEG-HZ-PE conjugate is purified using size exclusion chromatography using Sepharose CL4rB as described before. 

Figure 4-5 PNA-encoded self-assembling chemical arrays, (a) Each small molecule was labeled with a PNA tag as well as a fluorophore. (b) The tagged molecules were hybridized with their complementary sequences on a decoding array, (c) Incubation of the tagged molecule library with a protein sample generated a protein-small molecule conjugate, which could be separated by using size-exclusion chromatography. Structural information on the bound small molecule was decoded by the decoding array.

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