Size-exclusion chromatography broad molecular weight standard

Direct calibration of (gel permeation chromatography/size exclusion chromatography) GPC/SEC columns requires well characterized polymer standards of the same type of polymer one has to analyze. However, narrow molecular-weight distribution (MWD) standards are available for a limited number of polymers only, and well-characterized broad MWD standards are not always accessible. The parameter controlling separation in GPC/SEC is the size of solute in the chromatographic eluent. Therefore, if different polymer solutes are eluted in the same chromatographic system with a pure exclusion mechanism, at the same retention volume, molecules with the same size will be found. 

---