Size exclusion chromatographic detection

Brown, R. S., Hausler, D. W., Taylor, L. T., and Carter, R. C., Fourier transform infrared spectrometric detection in size-exclusion chromatographic separation of polar synfuel material, Anal. Chem., 53, 197, 1981. 

Figure 3. The size-exclusion chromatographic separation of a standard protein mixture on a Hibar RT LiChrospher 500 DIOL column. COhimn size, 250 X 4 mm (2 columns) eluant, 50 mM H3PO4 adjusted to pH 7.0 with 2.0 M NaOH -r 1% SDS flow-rate, 0.2 ml/min pressure, 15 kg/cm chart speed, 2.5 mnVntin detection, UV 208 nraO.08 AUFS. Samples, (1) albumin (bovine serum = 68 (KK)) (2) chymo-trypsinogen A (25 000) (3) cytochrome c (12 500) (4) alanine (89).

Simonsick, Jr., W. J. and Prokai, L., Size-exclusion chromatography with electrospray mass spectrometric detection, in Chromatographic Characterization of Polymers, Hyphenated and Multidimensional Techniques, Provder, T., Barth, H. G. and Urban, M. W., Eds., American Chemical Society, Washington, D.C., 1995, chap. 4. 

A way out of the dilemma is the determination of the molecular size distribution of the chromatographable organic carbon (COC) as it is offered from size exclusion chromatography (SEC) equipped with an organic carbon (OC) detection system. 

Figure 10.3. Size exclusion chromatogram obtained by UV and OC detection (SEC/UV-DOC) for a river sample (river Neckar, Heidelberg, 22.03.2007, DOC = 2.3 mg liter 1, chromatographable organic carbon (COC = 75%). Column TSK HW50S (250 x 20mm). Eluent phosphate buffer, 26.8mmol liter 1, UV (254nm) and organic carbon (OC) detection, exclusion volume V0, permeation volume Vp).

Liquid chromatographic analyses with ICP-MS detection may be divided into categories according to the mode of chromatography used reversed-phase, ion-pair, micellar, ion-exchange, and size-exclusion chromatography. 

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