Site Directed Unnatural

Caged Proteins by Introduction of Photoactive Residues via Site Directed, Unnatural Amino Acid Mutagenesis... 

Fig. 5 Proposed mechanism of the formation of unnatural polyketides by site-directed mutants of...

Cold-adapted mutants Site-directed mutants Gene-deleted mutants Gene reassessment mutants Natiwally available mutants Mutants generated by adaptation to unnatural host... 

Fleissner MR, Brustad EM, Kalai T, Altenbach C, Cascio D, Peters FB, Hideg K, Peuker S, Schultz PG, Hubbell WL (2009) Site-directed spin labeling of a genetically encoded unnatural amino acid. Proc Natl Acad Sci USA 106(51) 21637-21642... 

Improvements to protein expression. Whilst many of the advances in studying biomolecules by NMR are as a direct result of developments in sample labelling schemes, such as isotope enrichment at specific sites or unnatural amino add incorporation (yide infra), there are still great improvements that can be made to routine protein expression methods. For conventional and perdeuteration, Li et al. describe optimisation of... 

The attractiveness of this enzyme resides in its potential to catalyze self-aldol, cross-aldol, and cascade aldol reactions (for a review, see Ref. 87). DERA generally presents a stereoselectivity toward the aldehyde acceptor and catalyzes often the conversion of only one enantiomer. However, it often requires the presence of phosphorylated aldehyde acceptors. The most recent efforts were thus focused on directed evolution of the enzyme in view to improve the scope toward unphosphorylated substrates. DeSantis et al., in particular, performed some saturation site-directed mutagenesis in view to improve the reactivity for the unnatural D-glyceraldehyde substrate 37 for the synthesis of 2-deoxy-D-ribose 38. They reached a 2.5-fold improvement in DERA activity toward the unphosphorylated aldehyde with the mutant S238D (Scheme 28.18). 

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