Single Turnover Stopped-Flow Studies of Electron Transfer

SINGLE TURNOVER STOPPED-FLOW STUDIES OF ELECTRON TRANSFER 

FIGURE 4. Spectral forms of intermediates in the reductive half-reaction of TMADH. Panel A, photodiode array analysis of the reaction of TMADH with TMA. Panel B, Denconvoluted spectra for the intermediates of the reductive half-reaction. Spectrum A, oxidised enzymes spectrum B, reduced enzyme (dihydroflavin) spectrum C, reduced enzyme (flavin semi-quinone/reduced iron-sulfur centre) spectrum D, spin-interacting state. 

pH-Dependence of the Reductive Half-reaction with Diethylmethylamine 

The data thus reveal that ionizable groups are responsible for controlling the rate of flavin reduction. The intermediate and slow kinetic phases of the reductive half-reaction with diethylmethylamine were observed to be essentially independent of substrate concentration above pH 7 below 

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