Single strand break products

Millar BC, Sapora O, Fielden EM, Loverock PS (1981) The application of rapid-lysis techniques in radiobiology. IV. The effect of glycerol and DMSO on Chinese hamster cell survival and DNA single-strand break production. Radiat Res 86 506-514 Milligan JR, Ward JF (1994) Yield of single-strand breaks due to attack on DNA by scavenger-derived radicals. Radiat Res 137 295-299... 

DNA-DNA crosslinks develop with time following exposure to HgCl2, probably resulting from its ability to interact directly with the DNA bases [253], while single-strand breaks may result from the production of oxygen radicals by mercuric chloride and also by its interaction with DNA bases [254, 255], The single-strand breaks resemble those induced by X-rays [256]. 

LOMEFLOXACIN t UVA IS min Figure19.3 Useofsupercoiled plasmid circular DNA for detection of ROS production by photo-activated lomefloxacin. Form I supercoiled form (no DNA breaks) form II relaxed form (few DNA single strand breaks) form III linearform (high level of DNA single strand breaks leading to double strand breaks see [38]). 

Doxorubicin binds tightly to DNA by its ability to intercalate between base pairs and therefore is preferentially concentrated in nuclear structures. Intercalation results in steric hindrance, hence production of single-strand breaks in DNA and inhibition of DNA synthesis and DNA-dependent RNA synthesis. The enzyme topoisomerase II is thought to be involved in the generation of DNA strand breaks by the anthracydines. Cells in S-phase are most sensitive to doxorubicin, although cytotoxicity also occurs in other phases of the cell cycle. 

Folkard M, Prise KM, Vojnovic B, Brocklehurst B, Michael BD (2000) Critical energies for ssb and dsb induction in plasmid DNA by vacuum-UV photons an arrangement for irrdiating dry or hydrated DNA with monochromatic photons. Int J Radiat Biol 76 763-771 Folkard M, Prise KM, Turner CJ, Michael BD (2002) The production of single strand and double strand breaks in aqueous solution by vacuum UV photons below 10 eV. Radiat Prot Dosim 99 147-149 FoxRA, Fielden EM, SaporaO (1976) Yield of single-strand breaks in the DNA of E. coli 10 msec after irradiation. Int J Radiat Biol 29 391-394... 

Several natural products have been tested to determine their ability to disrupt the function of human DNA ligase I, which catalyzes the covalent joining of single-stranded breaks in double-stranded... 

Did not alter production of DNA single-strand breaks by y rays inhibited repair of these breaks in L7712 cells 633... 

Panajotovic R, Martin F, Cloutier P, Hunting D, Sanche L (2006). Effective cross sections for production of single- strand breaks in plasmid DNA by 0.1 to 4.7 eV electrons. Radiat Res 165 452-459. 

Reaction of H02 -Fe(m)Blm with DNA to produce a single strand break had been conceived of in two ways in the first, abstraction of the C4 hydrogen formally involves a species equivalent to a hydroxyl radical. As such, there is homolytic cleavage of the peroxide, leaving a ferryl species, equivalent to a hydroxyl radical and Fe(in)Blm, as the other product. 

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