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Single-site binding model

Figure 5.9 Typical ITC data for binding of a trisaccharide inhibitor (tri-/V-acetyl-glucosamine tri-NAG) to hen egg white lysozyme, in 0.1 mol dm ethanoate buffer, pH 5. Each exothermic heat pulse (upperpanel) corresponds to injection of 10 nL (0.01 cm ) of tri-NAG (0.45 mmol dm ) into the protein solution (36 nmol dm ). Integrated heat data (lower panel) constitute a differential binding curve that may be fit to a standard single-site binding model to give, in this instance, the stoichiometry of binding (number ol binding sites), A/=0.99 binding affinity. Figure 5.9 Typical ITC data for binding of a trisaccharide inhibitor (tri-/V-acetyl-glucosamine tri-NAG) to hen egg white lysozyme, in 0.1 mol dm ethanoate buffer, pH 5. Each exothermic heat pulse (upperpanel) corresponds to injection of 10 nL (0.01 cm ) of tri-NAG (0.45 mmol dm ) into the protein solution (36 nmol dm ). Integrated heat data (lower panel) constitute a differential binding curve that may be fit to a standard single-site binding model to give, in this instance, the stoichiometry of binding (number ol binding sites), A/=0.99 binding affinity.
For simple processes in which multiple metals bind to a single ligand = 1 and iEL = 1 (AEE1 = 0) and hence the extended site binding model reduces back the basic site binding model. Figure 10.17 shows... [Pg.646]

Let us consider the fixation of a bidentate ligand to a single metal as depicted in Figure 84. The first step simply corresponds to a standard intermolecular binding process modeled by a statistical factor and an absolute intermolecular affinity according to the site-binding model... [Pg.433]

In order to measure any constant, we need an equation that relates this constant to variables that maybe determined by experiment. For a single-site, single-affinity binding model, there is a relatively simple way of deriving such an equation. If we define total concentration of receptor [R]o according to... [Pg.341]

The fitting of ITC data requires a model to calculate the change in complex concentration for each incremental increase in ligand concentration. For example, for single-site binding with a binding constant K, the concentration of complex after the ith injection is given by ... [Pg.413]

It is surprising that data on natural particles can be fitted over a range of concentrations (representative of those encountered in natural waters) on the basis of a "single-site" surface complex formation model. Apparently similar types of binding groups are predominant and of importance in these particles. [Pg.378]

Finally, we consider the effects on recognition and binding of single site replacements [34] of the individual charged residues which have been emphasized in molecular modeling studies. These residues include Lys (18, 32, 77) in cytochrome c and Asp (37, 79, 217) in ccp. [Pg.172]

One of our main assumptions in the derivation of the Langmuir model [and implicitly made by Langmuir himself (1918)] is that the binding process does not affect the distribution of states of the adsorbent molecules. Removal of this assumption has a profound effect on the form of the BI of systems with more than a single site. [Pg.51]

Substituting these expressions into the equations above yields a new expression that enables the interaction kinetics to be readily modeled for single-site, reversible binding between a protein and a single ligand ... [Pg.144]


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See also in sourсe #XX -- [ Pg.236 ]




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Binding single-site

Single-site model

Site binding model

Site modeling

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