Single molecule multiparameter fluorescence detection

A sophisticated experimental setup capable of simultaneous measurement of intensity, lifetime and anisotropy of both the donor and acceptor dyes has been reported by Margittai et al. [11]. The advantage of this demanding approach which they have termed single molecule multiparameter fluorescence detection (smMFD) is that it overcomes the problems associated with simpler FRET schemes such as the bleaching of donor or acceptor (see also the ALEX technique Chapter 2 and [12,13]) and assumptions concerning the quantum yields and... 

Widengren, J., Kudryavtsev, V., Antonik, M., Berger, S., Gerken, M. and Seidel, C. A. (2006). Single-molecule detection and identification of multiple species by multiparameter fluorescence detection. Anal. Chem. 78,2039-50. 

C. Eggeling, S. Berger, L. Brand, J.R. Fries, J. Schaffer, A. Volkmer, C.A. Seidel, Data registration and selective single-molecule analysis using multiparameter fluorescence detection, J. Biotechnol. 86, 163 (2001)... 

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