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Single-molecule methods

Common to most fluorescence-based single-molecule methods, photobleaching limits the observation time window using the fluorescence quenching via energy transfer approach. In addition, this approach only obtains the fluorescence intensity from one probe thus, fluorescence intensity fluctuations due to probe photophysics, such as fluorescence blinking, can complicate the results and data analyses. Triplet quenchers such as Trolox 95 can reduce fluorescence blinking. Careful control experiments are in any case necessary. [Pg.764]

Topics covered include an Introduction to biological molecules spectroscopy, mass spectrometry and hydrodynamics of macromolecules a bluffer s guide" to molecular thermodynamics biomolecular kinetics chromatography and electrophoresis and single-molecule methods. [Pg.186]

The described methods are meanwhile applied to a multitude of different biological systems. For the following examples, we focus on applications of single-molecule methods to synthetic polymer and interface science. To our knowledge, there is no such application with MT and OT published yet, therefore, we give recent examples on the best studied biopolymer (DNA) for these two methods. [Pg.640]


See other pages where Single-molecule methods is mentioned: [Pg.369]    [Pg.253]    [Pg.50]    [Pg.165]    [Pg.61]    [Pg.139]    [Pg.436]    [Pg.751]    [Pg.752]    [Pg.753]    [Pg.766]    [Pg.134]    [Pg.213]    [Pg.205]    [Pg.2]    [Pg.73]    [Pg.80]    [Pg.245]    [Pg.246]    [Pg.249]    [Pg.61]    [Pg.36]    [Pg.86]    [Pg.629]    [Pg.629]    [Pg.629]   
See also in sourсe #XX -- [ Pg.369 , Pg.388 , Pg.389 , Pg.390 , Pg.391 , Pg.392 ]




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