Single mismatch discrimination

Detection of the bound PNA is possible via on-column fluorescence detection. Efficient single base-pair discrimination was achieved routinely using that technique, as shown in Fig. 7. The system was optimized to observed a difference of 25°C between the perfect match and single-mismatched PNA probe. The assay should be applicable for clinical research and diagnostics and may be automated as well. 

The single mismatch (SNP) discrimination capabDity of the experiment shown in Fig. 6f is due to hydrodynamic shear. In a recent MD project [15], it has been shown that shear is most discriminating because it can meter small thermal-energy-level... 

Considerable amounts of quenching of the acridone emissions by guanine in the DNA occurred when guanine was close to acridone, which can be applied as a quencher-free probe (no additional quencher is required) for the detection of a special sequence of DNA. The DNA bearing acridone at the C5 position of inner thymidine could distinguish the opposite T-T base mismatch, while enhancement of discrimination ability is needed for the practical use of single nucleotide polymorphism (SNP) typing. 

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