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Single cell tracking assays and analysis

Migration of individual leukocytes in vivo were recorded as early as the mid-1800s fascinating reviews of these early studies are available in which detailed accounts are given of individual leukocytes migrating in the rabbit cornea and ear, tadpole tails, and frog tongue and webs [63, 237]. [Pg.325]

Like population assays, single cell movement has also been measured in three-dimensional gel invasion assays [117,148, 247, 296]. These gels can be constructed such that the chemoattractant concentration gradients are well defined [151, 247]. [Pg.326]

Using current technology, computer-based image analysis methods are utilized to analyze data collected with fast ccd cameras allowing for high time resolution of the motion both in two dimensions and three dimensions [87, 247]. From measurements of ((Ax) ) and At the parameters S and P can be extracted. Theoretical treatment of the model and optimization of analysis methods have been reported [79, 80, 89, 224, 393]. [Pg.327]

Relationship between population and oell tracking data [Pg.328]


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