Simultaneous saccharification and co-fermentation

Simultaneous saccharification and co-fermentation (SSCF) one-stage enzymatic hydrolysis of cellulose and fermentation of pentoses and hexoses all in one process step. The upstream hydrolysis of the hemicellulose takes place in a separate process step. 

Zhang, J., Shao, X., Townsend, O.V., and Lynd, L.R. (2009) Simultaneous saccharification and co-fermentation of paper sludge to ethanol by Sac-charomyces cerevisiae RWB222—Part I kinetic modeling and parameters. BiotechnoL Bioeng., 104 (5), 920—931. 

Platform compounds are further converted to products by microbial fermentation processes. Saccharification and fermentation can be accomplished in a sequential process by separate hydrolysis and fermentation (SHF), or in a consolidated one-pot process known as simultaneous saccharification and fermentation (SSF) of single sugars or simultaneous saccharification and co-fermentation (SSCF) of all monosaccharides. Future developments might even combine the production of saccharolytic enzymes, the hydrolysis of cellulose, and hemicellulose to monomeric sugars and the fermentation of hexose and pentose sugars in a single process, the so-called consolidated biomass processing (CBP) (Menon and Rao 2012). 

Morales-Rodriguez, R., K. V. Gemaey, A. S. Meyer and G. Sin (2011a). A mathematical model for simultaneous saccharification and co-fermentation (SSCF) of C6 and C5 sugars. Chinese Journal of Chemical Engineering 19(2) 185-191. 

Acid-catalysed hydrolysis with steam explosion Simultaneous saccharification and co-fermentation (SSCF)... 

Hydrolysis and fermentation After the pretreatment steam is recovered in a flash drum before the slurry enters the simultaneous saccharification and co-fermentation (SSCF) reactor. The ethanol concentration of the slurry leaving the reactor is 4-5 wt%. 

Olofsson, K., Palmqvist, B., liden, G., 2010. Improving simultaneous saccharification and co-fermentation of pretreated wheat straw using both enzyme and substrate feeding. Biotechnology and Biofuels 3,17. 

Zhang, J., Lynd, L.R., 2010. Ethanol production from paper sludge by simultaneous saccharification and co-fermentation using recombinant xylose-fermenting microorganisms. Biotechnology and Bioengineering 107,235-244. 

Golias H, Dumsday GJ, Stanley GA, Pamment NB. (2002). Evaluation of a recombinant Kfeb-siella oxytoca strain for ethanol production from cellulose by simultaneous saccharification and fermentation comparison with native cellobiose utilizing yeast strains and performance in co-culture with thermotolerant yeast and Zymomonas mobilis. J Biotechnol, 96,155-168. 

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