Simazine soil analysis

Gong, A.J., C.M. Ye, Z.F. Lei, and J.A. Liu. (1999). Pre-column derivatization reaction coupled with ultrasonic wave extraction for the trace analysis of atrazine and simazine in soil and crops by liquid chromatography, ultraviolet detection. J. Liq. Chrom. Rel. Technol., 22 2535-2545. 

Triazines such as atrazine, propazine or simazine, are widely used herbicides. Currently, the determination of atrazine in water and soil samples is mainly done by GLC (1,2) or HPLC (3-5). However, these procedures require cumbersome cleanup steps which could be avoided by using immunoassays as an alternative approach to residue analysis. Such immunochemical determination based on competitive binding of herbicides or pesticides to an antibody (6,7), has been described recently for s-... 

EIA and GC results were compared for the analysis of simazine in soil, using ethyl acetate for extraction of dried samples of sandy loam from a site contaminated by an experimental simazine spill. For 24 samples that had simazine content between 0 and 350 ppb by GC analysis, the results by EIA correlated with r = 0.93 and a slope of 1.26 (Figure 6). For an extended data set of 48 samples containing simazine from 0 to 3 ppm, the correlation between EIA and GC determinations was 0.97, with a slope of 0.81 (A. Lucas, unpublished data). To ensure solubilization of the simazine recovered from the most heavily contaminated samples, methanol was added to the PBS>Tween to 5% (v/v) as coeolvent. Atrazine residues were recovered by extraction with 90% acetonitrile and concentration on SCX solid phase columns. For samples spiked with 10, 25, 50, and 100 ppb (ng atrazine per gram of soil) recoveries of 80%, 82%, 79%, and 93%, respectively, were obtained. 

Semiquantitative analysis of prometryne, simazine, and atrazine was carried out in soil samples by their Hill reaction inhibitory effect (167) (Table 12). A chronometric method was developed for the quantitative determination of these herbicides in food samples with recoveries between 70% and 100% (168). This method was extended for the determination of residues of atrazine, lenacyl, and chloridazon in commercial sugar samples with recoveries, 90,90, and 60%, respectively (169) (Table 12). 

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