Silver MALDI matrix-assisted laser desorption

A modified version of 2DE and gel image analysis, with silver staining, autoradiography, and protein identification and measurement of peptide mass, uses matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as a rapid and sensitive technique for identifying peptides. MALDI-TOF-MS applies well to protein detection in biological fluids.56 A second advantage of this technique is... [Pg.87]

Macha, S. Hanton, S. Owens, K. Limbach, P. Silver cluster interferences in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of non-polar polymers. J. Am. Soc. Mass Spectrom. 2001, 12.132-1 A3. [Pg.243]

The use of silver nanoparticles has also been described in matrix-assisted laser desorption/ionization (MALDI), a powerful laser-based soft ionization technique for mass spectrometric analysis and the investigation of peptides, proteins, nucleic acids, pharmaceuticals, bacterial characterization and imaging studies. Here, liquid-liquid microextraction base-modified silver nanoparticles were employed for the extraction of a hydrophobic peptide (gramicidin) from biological samples through hydrophobic interactions, prior to MALDI analysis (Figure 4.1) [41]. The application of silver nanoparticles was shown to provide an excellent sample cleanup procedure, and also assisted in the enhancement of signal of peptides and proteins. [Pg.153]