Sierra Analytics

Sierra Analytics and Applied Biosystems investigated the metabolic profile of indinavir, an HIV protease inhibitor with a very short half-life (2 hours), readily metabolised via CYPs to produce phase I and subsequent II products. Aliquots (10 pL) of indinavir incubated with rat liver S9 fractions were separated by online liquid chromatography and analysed with an Applied Biosystems hybrid triple quadrupole/linear ion trap mass spectrometer in information dependent acquisition (IDA) mode. This consisted of an enhanced MS survey scan followed by enhanced product ion scans for the two most intense parent ions as dependent MS/MS experiments. 

Figure 1.4. Phase diagram for the system K O-AfCVSiCVF O at 25 °C and 1 atmosphere. Open circles are analytical data for water from springs or seeps in a granitic terrain in the Sierra Nevada (from Feth and others) (%) (from Birkeland, 1984, after Feth and others, 1964. Reprinted from Soils and Geomorphology, pill, Copyright (1984), with permission from Oxford University Press, New York, Oxford)...

Galban J, Andreu Y, Sierra IF, de Marcos S, Castillo JR. Intrinsic fluorescence of enzymes and fluorescence of chemically modified enzymes for analytical purposes a review. Luminescence 2001 16 199-210. 

F. Sierra, G. Sanchez-Pedreno, T. P. Ruiz, and C. Martinez Lozano, Analyt. Chim. Acta, 1977,94,129. 

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