Sialooligosaccharides

Puromycin. Puromycin (19), elaborated by S. alboniger (1—4), inhibits protein synthesis by replacing aminoacyl-tRNA at the A-site of peptidyltransferase (48,49). Photosensitive analogues of (19) have been used to label the A-site proteins of peptidyltransferase and tRNA (30). Compound (19), and its carbocycHc analogue have been used to study the accumulation of glycoprotein-derived free sialooligosaccharides, accumulation of mRNA, methylase activity, enzyme transport, rat embryo development, the acceptor site of human placental 80S ribosomes, and gene expression in mammalian cells (51—60). 

Mass spectrometric investigations of derivatized sialooligosaccharide-alditols, sialoglycolipids, and sialoglycopeptides have been carried out by several authors. Because of the relatively low volatility of these substances, g.l.c. cannot in general be used as m.s. inlet system. In the framework of this chapter, only some examples will be mentioned. 

Figure 4. Building blocks for the systematic synthesis of sialooligosaccharides, gangliosides, and their derivatives and analogs. X = NHAc, NHCOCHjOAc, NHCOCFj, NHBoc, OAc, etc. Y = SMe, OC(= NH)CCl3, etc. P = Ac, Bz, etc.

Receptor Function of Gangliosides and Sialooligosaccharides for Bacteria, Viruses, and Bacterial Toxins... 

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